Volume 6, Issue 1, June 2020, Page: 7-13
The Cyclin Dependent Kinase Inhibitor Protein 21 Cytoplasmic Expression Depressing the Apoptosis of Human Bronchial Epithelial Cell
Zou Guoming, Respiratory and Critical Medical Department, Jiangxi Provincial People's Hospital, Nanchang, China
Xiao Zuke, Respiratory and Critical Medical Department, Jiangxi Provincial People's Hospital, Nanchang, China
Received: Jun. 15, 2019;       Accepted: Dec. 10, 2019;       Published: Jan. 8, 2020
DOI: 10.11648/j.ijcda.20200601.12      View  622      Downloads  122
Abstract
AIMS: To investigate the cytoplasmic P21 expression regulating the apoptosis of human bronchial epithelial cell. MOTHEDS: The relationship of the cytoplasmic P21 expression with the apoptosis of 16HBE cells was studied after the plasmid pEGFP-N1-P21 was transfected into the 16HBE cells. After the 16HBE cells was the stimulated by the TGF-β1, the cytoplasmic and nucleic P21 expression and the apoptosis of 16HBE cell was detected, then the relationship of the P21 expression with the apoptosis of 16HBE cells was studied. RESULTS: The 16HBE cell had the basic low cytoplasmic and mainly high nucleic P21 protein expression, the plasmid PEGFP-N1-P21 could express P21 protein only in the cytoplasm of 16HBE cell and did not affect the nucleic P21 protein level. The apoptosis of 16HBE cells after transfection of the PEGFP-N1-P21 decreased. The apoptosis of 16HBE cells decreased as the time of the PEGFP-N1-P21 transfection increased, but the apoptosis of 16HBE cells increased without the PEGFP-N1-P21 transfection. The stimulation by TGF-β1 led to the expression of the cytoplasmic and nucleic P21 proteins, but mainly the cytoplasmic P21 protein expression, as the stimulation concentration of TGF-β1 increased, the cytoplasmic P21 expression decreased, but the nucleic P21 did not change. The apoptosis of 16HBE cells increased as the cytoplasmic P21 expression decreased after the concentration of TGF-β1 stimulation increased. CONCLUSIONS: The apoptosis of 16HBE cell was inhibited by the high cytoplasmic P21 expression through the transfection of PEGFP-N1-P21. TGF-β1 stimulation promoted the apoptosis of 16HBE cell by inhibiting the cytoplasmic P21 expression. The cytoplasmic P21 expression depresses the apoptosis of 16HBE cells.
Keywords
Cyclin Dependent Kinase Inhibitor P21, Transforming Growth Factor-β1, Apoptosis
To cite this article
Zou Guoming, Xiao Zuke, The Cyclin Dependent Kinase Inhibitor Protein 21 Cytoplasmic Expression Depressing the Apoptosis of Human Bronchial Epithelial Cell, International Journal of Clinical and Developmental Anatomy. Vol. 6, No. 1, 2020, pp. 7-13. doi: 10.11648/j.ijcda.20200601.12
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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