Volume 6, Issue 1, June 2020, Page: 1-6
Chicken Cyclo-oxygenases Response in a Pulmonary Infection Disease
Aida Karray, National School of Engineers of Sfax ENIS, University of Sfax, Sfax, Tunisia
Sofiane Bezzine, National School of Engineers of Sfax ENIS, University of Sfax, Sfax, Tunisia
Received: Nov. 18, 2019;       Accepted: Dec. 21, 2019;       Published: Jan. 6, 2020
DOI: 10.11648/j.ijcda.20200601.11      View  153      Downloads  44
Avian infectious bronchitis, associated coronavirus causes inflammation and damage to the lungs. To evaluate the molecular mechanisms behind this event, we investigated variations in the expression level of the proinflammatory factors, cyclo-oxygenase 1 (COX-1) and cyclo-oxygenases 2 (COX-2). In mammals, the increased generation of prostaglandins during the onset of inflammatory responses and activation of immune cell types has been attributed to the induction of COX-2. The recent sequencing of chicken genome and the identification of genes coding COX-1 and COX-2 allowed us to analyze their expression in lung inflammatory disease. The purpose of this study was to examine the relative expression and distribution of COX-1 and COX-2 in organs of normal hens and in hens with avian infection bronchitis. The expression levels of cyclo-oxygenase genes in several organs from uninfected chickens and infected ones were measured using a quantitative reverse transcription-PCR technique. Our results show that the expression level of COX-1 was highly up regulated in spleen, small intestine, lungs, and liver, but modestly up regulated in the pancreas. However, no significant change was observed in the expression levels of COX-2, in the small intestine, liver and spleen; suggesting a constitutive expression of this enzyme. Whereas, COX-2 mRNA expression was significantly decreased in the heart and lungs of infected chickens, ranging from 9-fold to 4-fold, respectively (p<0.05). Contrary to mammals, COX-2 is present constitutively in bird’s cells, while COX-1 is expressed primarily after inflammation.
Cyclo-oxygenase, Gene Expression, qPCR, Gallus gallus, Avian Infectious Bronchitis
To cite this article
Aida Karray, Sofiane Bezzine, Chicken Cyclo-oxygenases Response in a Pulmonary Infection Disease, International Journal of Clinical and Developmental Anatomy. Vol. 6, No. 1, 2020, pp. 1-6. doi: 10.11648/j.ijcda.20200601.11
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Cavanagh D, N. S., Infectious bronchitis. Diseases of poultry, ed. t. ed. 2003: Ames, Iowa State Univer Pr. 101-119.
Hamaguchi, K., et al., Induction of distinct sets of secretory phospholipase A (2) in rodents during inflammation. Biochim Biophys Acta, 2003. 1635 (1): p. 37-47.
Chomczynski, P. and N. Sacchi, Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 1987. 162: p. 156-159.
Eskola, J. U., T. J. Nevalainen, and T. N. Lovgren, Time-resolved fluoroimmunoassay of human pancreatic phospholipase A2. Clin Chem, 1983. 29 (10): p. 1777-80.
Cavanagh D, G. J., Infectious bronchitis. 12th ed ed. Diseases of poultry,, ed. S. YM. 2008, Ames (IA): Wiley-Blackwell publishing. 101-120.
Wang, L., D. Junker, and E. W. Collisson, Evidence of natural recombination within the S1 gene of infectious bronchitis virus. Virology, 1993. 192 (2): p. 710-6.
Kujubu, D. A., et al., TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue. J Biol Chem, 1991. 266 (20): p. 12866-72.
Williams, C. S., M. Mann, and R. N. DuBois, The role of cyclooxygenases in inflammation, cancer, and development. Oncogene, 1999. 18 (55): p. 7908-16.
Miyamoto, T., et al., Purification of prostaglandin endoperoxide synthetase from bovine vesicular gland microsomes. J Biol Chem, 1976. 251 (9): p. 2629-36.
Hemler, M. and W. E. Lands, Purification of the cyclooxygenase that forms prostaglandins. Demonstration of two forms of iron in the holoenzyme. J Biol Chem, 1976. 251 (18): p. 5575-9.
DeWitt, D. L., Prostaglandin endoperoxide synthase: regulation of enzyme expression. Biochim Biophys Acta, 1991. 1083 (2): p. 121-34.
Cirino, G., Multiple controls in inflammation. Extracellular and intracellular phospholipase A2, inducible and constitutive cyclooxygenase, and inducible nitric oxide synthase. Biochem Pharmacol, 1998. 55 (2): p. 105-11.
Carstens, E., Report from the 40th meeting of the Executive Committee of the International Committee of Taxonomy of Viruses. 2009. p. 1571-1574.
Cavanagh, D., Severe acute respiratory syndrome vaccine development: experiences of vaccination against avian infectious bronchitis coronavirus. Avian Pathol, 2003. 32 (6): p. 567-82.
Liu, S. and X. Kong, A new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and non-vaccinated flocks in China. Avian Pathol, 2004. 33 (3): p. 321-7.
Pandey, M., et al., Overexpression of COX-2 gene in oral cancer is independent of stage of disease and degree of differentiation. Int J Oral Maxillofac Surg, 2008. 37 (4): p. 379-83.
Dubois, R. N., et al., Cyclooxygenase in biology and disease. Faseb J, 1998. 12 (12): p. 1063-73.
Urick, M. E. and P. A. Johnson, Cyclooxygenase 1 and 2 mRNA and protein expression in the Gallus domesticus model of ovarian cancer. Gynecol Oncol, 2006. 103 (2): p. 673-8.
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