Histological Study of the Effect of Bioactive Glass on Tibial Bone Repair in Rats

Gamal Abdel Salam; Ali M. Ali; Osama F. Ahmed; Ibrahim S. Ali

doi:doi:10.11648/j.ijcda.20150102.11

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Histological Study of the Effect of Bioactive Glass on Tibial Bone Repair in Rats

Gamal Abdel Salam, Ali M. Ali, Osama F. Ahmed, Ibrahim S. Ali

Published in International Journal of Clinical and Developmental Anatomy (Volume 1, Issue 2)

Received: 26 May 2015 Accepted: 3 June 2015 Published: 11 June 2015

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Abstract

The main goal of this study was to histologically evaluate the healing of surgically created defects on the tibiae of adult rats after implantation of bioactive glass. Twenty adult Wistar rats (body weight of 300g) were divided into two groups: bioglass treated group (n=10) and control group (n=10). Unicortical bone defects with 3-mm diameter were performed in both tibiae of the animals and filled with bioglass particles. The rats were then sacrificed at 14, 30 , 60 and 90 days, and the tissues were prepared for histological processing, sectioning, and staining with hematoxylin and eosin, as well as Mallory trichrome, and analyzed under light microscope. Within 7-14 days, both groups presented connective tissue septa with new bone formation, more intense in bioglass treated group. In the subsequent periods (30, 60 and 90 days), these groups presented more mature bone tissue around the glass particles. Bone trabeculae formed in all experimental periods were juxtaposed to the glass particles. It can be concluded that bioglass materials promoted bone formation over the entire extension of the defect, independently of the size of the granules, thus confirming their biological osteoconductive property.

Published in	International Journal of Clinical and Developmental Anatomy (Volume 1, Issue 2)
DOI	10.11648/j.ijcda.20150102.11
Page(s)	13-21
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Bioglass, Albino Rat, Tibial Repair, Bone Healing

References

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APA Style

Gamal Abdel Salam, Ali M. Ali, Osama F. Ahmed, Ibrahim S. Ali. (2015). Histological Study of the Effect of Bioactive Glass on Tibial Bone Repair in Rats. International Journal of Clinical and Developmental Anatomy, 1(2), 13-21. https://doi.org/10.11648/j.ijcda.20150102.11

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ACS Style

Gamal Abdel Salam; Ali M. Ali; Osama F. Ahmed; Ibrahim S. Ali. Histological Study of the Effect of Bioactive Glass on Tibial Bone Repair in Rats. Int. J. Clin. Dev. Anat. 2015, 1(2), 13-21. doi: 10.11648/j.ijcda.20150102.11

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AMA Style

Gamal Abdel Salam, Ali M. Ali, Osama F. Ahmed, Ibrahim S. Ali. Histological Study of the Effect of Bioactive Glass on Tibial Bone Repair in Rats. Int J Clin Dev Anat. 2015;1(2):13-21. doi: 10.11648/j.ijcda.20150102.11

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@article{10.11648/j.ijcda.20150102.11,
  author = {Gamal Abdel Salam and Ali M. Ali and Osama F. Ahmed and Ibrahim S. Ali},
  title = {Histological Study of the Effect of Bioactive Glass on Tibial Bone Repair in Rats},
  journal = {International Journal of Clinical and Developmental Anatomy},
  volume = {1},
  number = {2},
  pages = {13-21},
  doi = {10.11648/j.ijcda.20150102.11},
  url = {https://doi.org/10.11648/j.ijcda.20150102.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcda.20150102.11},
  abstract = {The main goal of this study was to histologically evaluate the healing of surgically created defects on the tibiae of adult rats after implantation of bioactive glass. Twenty adult Wistar rats (body weight of 300g) were divided into two groups: bioglass treated group (n=10) and control group (n=10). Unicortical bone defects with 3-mm diameter were performed in both tibiae of the animals and filled with bioglass particles. The rats were then sacrificed at 14, 30 , 60 and 90 days, and the tissues were prepared for histological processing, sectioning, and staining with hematoxylin and eosin, as well as Mallory trichrome, and analyzed under light microscope. Within 7-14 days, both groups presented connective tissue septa with new bone formation, more intense in bioglass treated group. In the subsequent periods (30, 60 and 90 days), these groups presented more mature bone tissue around the glass particles. Bone trabeculae formed in all experimental periods were juxtaposed to the glass particles. It can be concluded that bioglass materials promoted bone formation over the entire extension of the defect, independently of the size of the granules, thus confirming their biological osteoconductive property.},
 year = {2015}
}

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TY - JOUR
T1 - Histological Study of the Effect of Bioactive Glass on Tibial Bone Repair in Rats
AU - Gamal Abdel Salam
AU - Ali M. Ali
AU - Osama F. Ahmed
AU - Ibrahim S. Ali
Y1 - 2015/06/11
PY - 2015
N1 - https://doi.org/10.11648/j.ijcda.20150102.11
DO - 10.11648/j.ijcda.20150102.11
T2 - International Journal of Clinical and Developmental Anatomy
JF - International Journal of Clinical and Developmental Anatomy
JO - International Journal of Clinical and Developmental Anatomy
SP - 13
EP - 21
PB - Science Publishing Group
SN - 2469-8008
UR - https://doi.org/10.11648/j.ijcda.20150102.11
AB - The main goal of this study was to histologically evaluate the healing of surgically created defects on the tibiae of adult rats after implantation of bioactive glass. Twenty adult Wistar rats (body weight of 300g) were divided into two groups: bioglass treated group (n=10) and control group (n=10). Unicortical bone defects with 3-mm diameter were performed in both tibiae of the animals and filled with bioglass particles. The rats were then sacrificed at 14, 30 , 60 and 90 days, and the tissues were prepared for histological processing, sectioning, and staining with hematoxylin and eosin, as well as Mallory trichrome, and analyzed under light microscope. Within 7-14 days, both groups presented connective tissue septa with new bone formation, more intense in bioglass treated group. In the subsequent periods (30, 60 and 90 days), these groups presented more mature bone tissue around the glass particles. Bone trabeculae formed in all experimental periods were juxtaposed to the glass particles. It can be concluded that bioglass materials promoted bone formation over the entire extension of the defect, independently of the size of the granules, thus confirming their biological osteoconductive property.
VL - 1
IS - 2
ER -

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Author Information

Gamal Abdel Salam

Anatomy Department, Benha Faculty of Medicine, Benha University, Benha City, Egypt
Ali M. Ali

Anatomy Department, Benha Faculty of Medicine, Benha University, Benha City, Egypt
Osama F. Ahmed

Anatomy Department, Benha Faculty of Medicine, Benha University, Benha City, Egypt
Ibrahim S. Ali

Faculty of Public Health and Health Informatics, Umm al- Qura University, Makah City, Saudi Arabia

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APA Style

Gamal Abdel Salam, Ali M. Ali, Osama F. Ahmed, Ibrahim S. Ali. (2015). Histological Study of the Effect of Bioactive Glass on Tibial Bone Repair in Rats. International Journal of Clinical and Developmental Anatomy, 1(2), 13-21. https://doi.org/10.11648/j.ijcda.20150102.11

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ACS Style

Gamal Abdel Salam; Ali M. Ali; Osama F. Ahmed; Ibrahim S. Ali. Histological Study of the Effect of Bioactive Glass on Tibial Bone Repair in Rats. Int. J. Clin. Dev. Anat. 2015, 1(2), 13-21. doi: 10.11648/j.ijcda.20150102.11

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AMA Style

Gamal Abdel Salam, Ali M. Ali, Osama F. Ahmed, Ibrahim S. Ali. Histological Study of the Effect of Bioactive Glass on Tibial Bone Repair in Rats. Int J Clin Dev Anat. 2015;1(2):13-21. doi: 10.11648/j.ijcda.20150102.11

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@article{10.11648/j.ijcda.20150102.11,
  author = {Gamal Abdel Salam and Ali M. Ali and Osama F. Ahmed and Ibrahim S. Ali},
  title = {Histological Study of the Effect of Bioactive Glass on Tibial Bone Repair in Rats},
  journal = {International Journal of Clinical and Developmental Anatomy},
  volume = {1},
  number = {2},
  pages = {13-21},
  doi = {10.11648/j.ijcda.20150102.11},
  url = {https://doi.org/10.11648/j.ijcda.20150102.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcda.20150102.11},
  abstract = {The main goal of this study was to histologically evaluate the healing of surgically created defects on the tibiae of adult rats after implantation of bioactive glass. Twenty adult Wistar rats (body weight of 300g) were divided into two groups: bioglass treated group (n=10) and control group (n=10). Unicortical bone defects with 3-mm diameter were performed in both tibiae of the animals and filled with bioglass particles. The rats were then sacrificed at 14, 30 , 60 and 90 days, and the tissues were prepared for histological processing, sectioning, and staining with hematoxylin and eosin, as well as Mallory trichrome, and analyzed under light microscope. Within 7-14 days, both groups presented connective tissue septa with new bone formation, more intense in bioglass treated group. In the subsequent periods (30, 60 and 90 days), these groups presented more mature bone tissue around the glass particles. Bone trabeculae formed in all experimental periods were juxtaposed to the glass particles. It can be concluded that bioglass materials promoted bone formation over the entire extension of the defect, independently of the size of the granules, thus confirming their biological osteoconductive property.},
 year = {2015}
}

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